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inducible tet plko neo  (Addgene inc)


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    Structured Review

    Addgene inc inducible tet plko neo
    Inducible Tet Plko Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inducible+tet+plko+neo/Tet-pLKO-neo+(Plasmid+%2321916)/pmc11385192-474-20-22
    Average 94 stars, based on 62 article reviews
    inducible tet plko neo - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    shRNA:

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage.
    Article Snippet: .. Generation of genetically engineered RPE1 cells Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with shTP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1mg/mL polybrene (Sigma-Aldrich).

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage
    Article Snippet: .. Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with sh TP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1 mg/mL polybrene (Sigma-Aldrich).

    Over Expression:

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage.
    Article Snippet: .. Generation of genetically engineered RPE1 cells Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with shTP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1mg/mL polybrene (Sigma-Aldrich).

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage
    Article Snippet: .. Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with sh TP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1 mg/mL polybrene (Sigma-Aldrich).

    Construct:

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage.
    Article Snippet: .. Generation of genetically engineered RPE1 cells Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with shTP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1mg/mL polybrene (Sigma-Aldrich).

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage
    Article Snippet: .. Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with sh TP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1 mg/mL polybrene (Sigma-Aldrich).

    Clone Assay:

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage.
    Article Snippet: .. Generation of genetically engineered RPE1 cells Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with shTP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1mg/mL polybrene (Sigma-Aldrich).

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage
    Article Snippet: .. Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with sh TP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1 mg/mL polybrene (Sigma-Aldrich).

    Plasmid Preparation:

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage.
    Article Snippet: .. Generation of genetically engineered RPE1 cells Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with shTP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1mg/mL polybrene (Sigma-Aldrich).

    Article Title: Human aneuploid cells depend on the RAF/MEK/ERK pathway for overcoming increased DNA damage
    Article Snippet: .. Lentiviral preparation of TP53 shRNA and MEK overexpression constructs were obtained by transfecting lentiviral packaging vectors (pMDL, pRev, VSVG), and inducible Tet-pLKO-neo (Addgene #21916) cloned with sh TP53 or pHAGE-MAP2K1 plasmid (Addgene #116757) in HEK293T cells, using JETPei® (Polyplus) following the manufacturer’s protocol. .. Lentiviral preparation was collected and RPE1-hTERT cells were transduced with 1 mg/mL polybrene (Sigma-Aldrich).



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